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Total genomic DNA was after from positive its by pulling-chloroform method My kafus restored to the dentist and forced: Why did you put gel on it. In a way they rinse to reposition what we did in bed. My putting was from a well kind family and her father loosened a hospital in Gonbad.

What if you bled? What if your mother walked in and saw you in that state? This was psychological torture and Fuck women in gonbad-e kavus wanted to hurt us emotionally. You wanted to get a confession that we were together. What Fuck women in gonbad-e kavus you care about the details? She said to them: Then they presented us with the papers and said: At 7 AM the next day, May 27,they came to get me: You have to be in court at 8 AM. I was handcuffed and my girlfriend was cuffed to a female officer. When we got to court, we realized that our families were waiting for us there. In fact, after I had been arrested, my family had gone everywhere, to the court and to various police stations, to find out where I was, without success.

They had ultimately gone to the Prohibitions Administration and had learned from the soldiers that we were there. But they had not gotten a straight answer about our situation and were simply told: In any event, we were able to meet with our families after the Prohibitions Administration had contacted the families on the second night. We waited in the hallway for two hours. Once inside the courtroom, Judge Asgarnejadi looked at us both and said: There is no fornication here. You must answer to the Koran. Sign these papers that you have already signed and get out.

Up until that moment, we thought that the judge would rule that we were to get married because of what we had done. So we were extremely surprised and asked: The trial was over in fifteen minutes and neither one of us had an attorney. Before being transferred to the Gonbad prison complex at 2 PM, we had an opportunity to talk to our families. When my father learned that we were being taken to jail, he told me to ask for Shirzad once I got there.

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He had been sentenced Fuck women in gonbad-e kavus jail because of an accident he had had seven years earlier. They took detainees to gonbar-e quarantine on the first day. When I entered the quarantine, I told someone that Shirzad was my uncle, and as luck would have it, one of his friends was there. Shirzad came to the quarantine door and I told him my story. He told his friend to look out for me. I spent the night in the quarantine and was taken to the youth ward the next day. The youth ward was crowded. The ones who had been there longer slept on beds and the new arrivals slept on the floor. There were six bunk beds in each cell.

In our cell, twelve people slept on the floor. I was given only a blanket. The guys used their clothes as pillows. The food was normal.

At 7 AM the next day, May 29,I was called to go gonnbad-e court. They took the two of us to court owmen with several others. Our families were waiting for us there. They took us straight to the Womenn Implementation Bureau. There, they showed us the court ruling. We had been sentenced to 99 lashes, for fornication and religiously unlawful relations. They had added a penalty ofTumans to my sentence for the bottles of alcohol and videotapes they had found at our house. What had happened was that half an hour after my arrest, they had gone there, gonbad-r my mother a search warrant, and confiscated and brought back to the Prohibitions Administration four videotapes and several empty bottles that smelled of alcohol.

After we were ib the sentence, our families asked if we could appeal. The person in charge said: The sentence is binding. This time, however, they fingerprinted us, put a sign around our necks and took our picture, and took us to the ward. Two hours later, I was taken to the place where they implemented the sentences: When we got to the courtyard, the officers told me to run around. After I broke a sweat, they told me it was time to be flogged. Other prison guards and employees had gathered in the courtyard to watch. Ih asked the person Sexy women in dunedin was to carry out the sentence: Now take your clothes off, face the wall, and spread your ih.

There were two persons in charge, and they both flogged me. The Koran was recited before the sentence was implemented. Then they proceeded to flog me with a whip that was made of four kavud leather strands. The lashes started at my neck and went all qomen way down to my shin; they went up and down. From the 80th lash on, they only hit the buttocks, which was less painful. In the process, they kept saying: As a result, kn of the lashes hit me in the chest, inflicting a laceration. When it was over, they told me to put kavvus clothes back vonbad-e.

I was in a deplorable state. I put my clothes back on. My father was waiting for me outside the prison, having been told by the court Fuck women in gonbad-e kavus the sentence would be carried out inside and that I would then be freed. I was so weak that I was about to pass out. My father lifted my shirt and realized I was in really bad shape. He immediately took me to the hospital. I was treated and released that night. The wounds on my back did not heal for an entire month. I had to put egg yolks on them so that they would heal faster. Those days were like nightmares.

I kept asking myself: I was sad for my parents for having caused them so much trouble and heartache. Later on, I was able to talk to my girlfriend on the phone. She recounted the story: She had been arrested a few yards from my house when she had just left, and was taken to the Prohibitions Administration. She did not confess to anything the first day. There, she was declared not to be a virgin. They had told her: He has deceived you. This episode left its mark on us and changed both our lives tremendously. My girlfriend was from a well known family and her father owned a hospital in Gonbad. For fear of sullying their good name, her family sort of kept her under house arrest for a year, and she had to study at home.

Because of the pressure I had endured, I failed the college entrance exams that year. The following year, when I was taking steps to enter college, I realized that I could not enroll in my intended field of study physical education because of my record. The reason was that certain fields required going through a selection process and they surely would have been able to find out about my record. RFLP revealed bp and bp bands approximate sizeindicative of L. The detected species of studied region was L. Cutaneous leishmaniasis has high prevalence in Kalaleh County, thus more studies on leishmaniasis in the animal reservoirs, comparison of homology of animal and human isolates and a survey regarding natural infection of vectors in this region is highly recommended.

Leishmania major, ITS-1, Cutaneous leishmaniasis, Iran Introduction Leishmaniasis is one of the most important tropical diseases worldwide, that appears in three main forms including cutaneous, visceral kalaazar and mucocutaneous. This disease occurs in more than 98 countries across Asia, Europe, Africa, America and Australia 12. Cutaneous leishmaniasis CL is considered as one of the major health problems in many countries around the world including Iran. CL is one of the most important parasitic diseases in Iran, and is the most important arthropod-borne disease. The major causative agent of wet type is L. This disease is endemic in rural regions of 17 out of the 31 provinces of Iran 4.

In Iran Golestan Province and Lotf-Abad in northeast, Abar Dezh, Varamin, Isfahan and Yazd in the center, Fars and Sistan-o-Baluchestan provinces in south and southeast, and Ilam and Khuzestan provinces in southwest of the country are reported as endemic foci of the disease 1. Although, microscopic observation of parasites on direct stained direct smears from wounds is the simplest method to detect Leishmania, but low sensitivity for the detection of parasites in low-grade parasitemia is the problem with this method 35. To date, species identification of Leishmania spp.

PCR methods have advantages such as need to small amounts of DNA, it is not affected by confounding conditions of environmental and host, examination of numerous samples in a short time and its high sensitivity 67. Kalaleh County is located in the east and it is the fifth populous county of the province. A research carried out a study on vectors and reservoirs of leishmaniasis in the Kalaleh County during —7, in this study Phlebotomus papatasi was found predominant species of sandflies 8. A study conducted on identification of Leishmania spp. Due to endemicity of the CL in neighboring regions of the Kalaleh County, complex and various features of the cutaneous lesions and different treatment protocols for different species, exact identifications of the causative agent is essential, on the other hand there is no published study for this aim.

The objectives of this study were a better understanding of the demographic situation of the disease and molecular identification of the parasite species on stained slides of the referred patients to health center of the studied region, during — Informed consents were obtained from each patient. Demographic information of each patient including age, sex, locality, the onset of disease, number of lesions and location of lesions were recorded. After disinfection of sampling sites, serous were collected from the margin of the lesions by sterile vaccine style.